Lab Handbook

Allgemeine Laboratoriumsordnung Anna's Protocols and Recipes Autoclaving

Building Maintenance

Cell Culture Facility Centrifugation Checklists for New and Leaving Group Members Chemicals, fine chemicals Confocal Microscope Copying & scanning

DeNovix DS-11 Spectrophotometer Dry ice

Electrical Safety Email addresses Emergencies

First Aid Fridges, cold-rooms, freezers

Gas Alarms Gases Glassware

Holidays Housing Rooms Hygiene Plan S1 / S2

IncuCyte Isotope Lab

K12 Bacteria Keys and magnetic cards

Last person's duties Leica CM1950 Cryostat Library

MEA Multi-Electrode Array Media for Bacteria culture Mineral water

Nitrogen tanks for storage of cells

Office supplies

PCR pH Meter Phosphor Imager Plate Reader

Repairs Responsibilities

Safety: S1, S2 Seminar room SnapGene Spectrophotomer

Telephone, fax Telephone lists

Vacuum pumps

Waste disposal Workshops

ZEISS Axiocam ZEISS Axiophot

AAVs

Rules and Regulations regarding Adeno associated Viruses: AAV German AAV English

Allgemeine Laboratoriumsordnung

General Laboratory Regulations Englisch

Anna's Protocols and Recipes

Anna's collection of protocols and recipes can be found under the following links:

Protocols:
https://1drv.ms/u/s!Ai6uLdHu6cZ9dMLpLG8xUnPnmhE?e=RzyN39
Recipes:
https://1drv.ms/u/s!Ai6uLdHu6cZ9fonJuBmOiDR10lU?e=yiIsgZ

Please don’t hesitate to let Anna know if you have suggestions for improvements/additions!!!

Autoclaving

Solid items (pipette tips, forceps etc.) and solutions are autoclaved by the Cleaning Kitchen staff. Everything you want to be autoclaved has to be labelled with autoclave tape, signed (readably) with your name and lab number. If you want any special treatment you have to write that as well. It will be finished and delivered the next day to your lab.

Building Maintenance - Theoretikum

http://www.zbt.uni-heidelberg.de/hausverwaltung/

Cell Culture Facility

Glassware which was in contact with cells has to be put into the steel container for autoclaving.

The other glassware (only media, PBS, etc. but no cells) has to be rinsed and put in the container for the cleaning kitchen.

Pasteur pipettes go into the buckets for overnight treatment in 2% Lysoformin.

Disposable items (tissue, tips, dishes etc.) which have been in contact with cells have to be put into autoclave bags. Full bags have to be closed and labelled with autoclave tape. They are stored for autoclaving in the steel container.

Cell suspensions or supernatants have to be sucked into the liquid waste bottle. Full bottles have to be closed and labeled with autoclave tape. They are stored for autoclaving in the steel container. Empty bottles get Lysoformin (for 2% final concentration).

Clean and sterile cells which have to be disposed should be sucked into the liquid waste bottle. The plastic-ware goes into the autoclave bag.

Contaminated dishes are incubated with Lysoformin for 4h in the Tissue culture pre-room at the sink. Do not forget to dispose of your plastic-ware after Lysoformin treatment.

Never enter the cell culture room if an incubator is open.

The Group leader is responsible for all actions of members of his group in the Cell Culture Facility and bears the sole responsibility for ensuring that all members of his group are instructed in safe cell culture techniques.
Users must be aware that any Cell Culture Facility operates at the level of its least careful user. Since members of all groups perform long term tissue culture on irreplaceable material, such as hybridomas or stably transfected cell lines, this means that all users must perform their routine cell culture as if they were working with material that took months of work to produce.
Disinfect your hands before and after work.
All hoods are available on a sign-up basis. Before and after use they have to be swabbed down with 70% Ethanol and left clean and entirely empty (except for the Bunsen burner and pipettes). The tubing of the vacuum pump has to be rinsed with 70% Ethanol. If a user finds the hood inadequately cleaned, the person previously recorded as using the hood will be responsible for cleaning it.
All tissue culture plastic in the incubator has to be labelled with your initials, the cell line, passage number and date. Each user has the responsibility to check his cells every second day for contamination. Unlabelled dishes will be removed immediately. Spillover in the incubator has to be removed instantly.
The Baker hoods use extensive air circulation. This means that volatile substances can accumulate inside the hood, where an explosive mixture is easily formed. It is extremely dangerous to use portable camping gas burners in the hood for this reason. It is also advisable to minimize exposure of open vessels containing volatile liquids such as ethanol. Never block the grid of the hood because you will break the flow of the hood.
Waste treatment:
Contamination with yeast, mould or bacteria:
Never open a window in the cell culture facility.
Opened bags with tissue culture plastic-ware have to be closed with Tesa colour tape and put back into the cupboard.
At the moment S2 work is allowed and carried out only in the Viruslab/hotlab in 364 and in Room 244 in 345.

If you are the last person to use this facility

- Make sure that the gas is turned off

- Switch off blower

- Switch off the normal light in the hood

- Switch off the centrifuge, leave the lid open

- Switch off the water-bath if you used it after 8.00 pm and no one booked the cell culture

If you use the tissue culture for the first time you have to be introduced to the rules by Claudia or Ursula.

Centrifugation

How to use the cooling- and ultra-centrifuges and rotors

1.) Ask if you don't know how to use any centrifuge or rotor before you start to work!

2.) Sign in the booking calendar and write down how long and how often you will use the centrifuge.

3.) Book the rotor that you need for the centrifuge.

4.) Before you use the centrifuge make sure that it is working and clean. If it is not clean, ask the one who used it before to clean it.

5.) Sign in the rotor- and centrifuge-book (only for ultra-centrifuges).

6.) After you used the centrifuge make sure that it is clean and leave the lid open when you switch off the centrifuge.

7.) Remove the rotor from the centrifuge.

The fuses in 345 blow sometimes – the fusebox is in room 242.

Handbooks:

345, corridor 293b:

Beckman_Optima TL 100_Ultracentrifuge (Handbook for TLX model with higher top speed)

Beckman_Avanti J-25_Centrifuge [ Quick Reference ]

Beckman_Optima_L-70K_Ultracentrifuge [ Quick Start Guide ]

366: ?

Maintenance of centrifuges

1.) Centrifuge and rotors always have to be clean.

2.) The thread of the rotor should always be clean and a very tiny bit greasy – use the grease in a very minimal way (Spinkote by Beckman, in a tube). **

3.) The O-rings of rotors and doors need a tiny amount of Beckman vacuum grease, in a small pot.**

4.) If anything happens, which seems not to be normal contact the person responsible.

** 2) and 3) normally performed every 6 months.
Heating to 30° and pumping (table-top models: for 1h) to remove any water in the vacuum oil performed by the person responsible.

Photocopiers/scanners in 345 & 366

The copiers/scanners offer multi-page double-sided b/w copying and colour scanning.

To use the copier enter the code number of your group which is written on the wall.

345: opposite room 207a. Paper and toner on the shelves in the connection corridor 293b opposite room 204

366: room 146. Paper on the floor under the copier, toner on the shelf.

To scan, ask Alan for help. Your scan will be sent to you by e-mail. Your e-mail address can be stored in the copier if you ask Alan first.

345/366 copier administration

Recycling of old toner cartridges: the easiest is to take them to the Ricoh copy room in the basement of the URZ, INF 293

University library

copy card from the Secretary.

DKFZ

copy card from the Secretary.

DeNovix DS-11 Spectrophotometer

366 room 123

Handbook
Software updates (Android version) ('Automatic Updater' on the machine itself not working)

Our Android-based model is no longer produced, so there will probably be no more updates after May 2020.

Users' files can be saved to a USB stick or sent by e-Mail.

Data can be printed.

Handbook here or on the device.

Admin password: 2015

Files backed up for 60 days on the Nbio ftp server: ftp://Readonly:345364@ftp.nbio.uni-heidelberg.de (Filezilla client recommended)

Dry ice

is stored in the 80°C upright freezer in the freezer room.If you had something delivered in dry ice, put the dry ice into one of the polystyrene boxes in the 80°C upright freezer in the freezer room and dispose of your polystyrene box either in the waste-wagon outside the institute or if taken back from the company (Roche, Fermentas, Invitrogen, NEB) in the area next to the paper garbage cart.

Electrical Safety

Since 2019 this is carried out by the Electronics Workshop http://www.zbt.uni-heidelberg.de/elektronik/elektrischepruefung.html

Previously performed by Alan Summerfield:

Basis checklist.xls

Basis Excel checklist

Elektropruefung

Elekropruefung

Email addresses

Anyone with a Uni-ID has the address: @uni-heidelberg.de

Alan Summerfield (room 204, INF 345) can give you an e-Mail address in the form Your_Family_Name@nbio.uni-heidelberg.de

This will be included in the Personnel Contact List and the appropriate Mailing List.

Emergencies

Full list of emergency telephone numbers

here

Mobile phones:

Uni phones:

Doctor, Police

110

Fire

112

Technische Leitwarte (monitoring of freezers)

06221 54-7272

7272

Technical emergency (lift stuck, water leak etc)

06221 54-4444

4444

In the event of an alarm, the gas supply valves are closed. To re-open them, see

Re-opening the gas supply valves

First Aid

First aid box in 345 opposite room 204.

First aid box in 366: ?

Any accident has to be reported to the Sekretariat and entered in one of the accident books near the first aid boxes.

Instruction videos (in English) for the AED (Automated External Defibrillator) that is in the hallway outside the door from 366 to 346.

https://www.youtube.com/watch?v=dkv2BrgLKa4

https://www.youtube.com/watch?v=rkzXO2YOvHM

Anleitungsvideos (auf Deutsch) für den AED (Automatisierter externer Defibrillator), der sich im Flur vor der Tür von 366 nach 346 befindet.

https://www.youtube.com/watch?v=MnVHXuIH0MI&t=4s (nicht genau das gleiche Gerät, aber sehr ähnlich)

https://www.youtube.com/watch?v=S1WPk2t2d-4

Fridges, cold-rooms and freezers

In case a fridge, cold-room or freezer breaks down, refer to the

Equipment and Responsibilities

page.

Gas Alarms

There are sensors monitoring the air quality in rooms 150 (nitrogen tank) and 154 (gas supply).

Room 150

The sensor monitors the level of nitrogen in the air.

When the sensors on the south-side wall detect a high nitrogen concentration in the room, the alarm is activated and the Leitwarte is notified. Open the doors to allow the excess nitrogen to escape and then fix the cause of the alarm. The ALARM RESET button is next to the north-side door.

Room 154

The 2 sensors monitor the levels of oxygen and carbon dioxide in the air.

When the levels rise or fall dangerously, the gas supply pipes for both CO₂ and O₂ are closed and the red warning lights inside the room and in the corridor will flash. If the CO₂level reaches 1.5% the alarm sounds outside 154 and the Leitwarte is informed. The alarm will continue to sound until the RESET button is pushed.

When the alarm sounds, it is not allowed to enter the room until the air concentration has gone back to normal. This will happen when the door has been opened and both ALARM lights on the wall console have gone off. The levels can be seen on the display inside the room. When a person enters the room, a second person MUST be outside the room to come to the aid of the first person, if necessary. Press the RESET button when ALARM_1 has gone off and the audible alarm will be silenced.

With the door open(!) fix the cause of the alarm.

Download the Bedienungsanleitung MCS 4000
here
.

Re-opening the gas supply valves
(also after a fire alarm, when the valves will have been turned off automatically)

In all cases, the gas supply valves must be opened by hand:

Go to the wall console on the left. If a red light is flashing to show that the valves are closed, then
Press the RESET button and the light will turn green.
Turn the key to OFF then ON to reset the console and open the gas valves (listen for the click) and the two lamps labelled CO₂ and O₂ will be green.
If there is a malfunction in a gas valve, its light on the console will be red.

'K1' and 'K2' refer to the solenoids / Magnetventile in the vertical gas pipes on the wall.
Download the instruction book for the Gas Commander MV (gas supply control)
here
.
There is sometimes a false alarm. The red 'O2 Mangel' warning sign in the corridor flashes and the LED next to Alarm 1 is lit but the gas lines solenoids do not appear to be closed.

In case of problems with the electronic monitoring equipment, contact Klinik Technik GmbH

On the touch screen next to the department's main door, push the flashing warning symbol at the top and ACK ('Acknowledge', confirm) to reset the alarm at the Leitwarte.

An alarm event log can be viewed here.

Gases

Safety information (Word document download)

Gas store webpage

Bottled gases

Often the reserve bottle is used before the old bottle has reached '0', so check the pressure in the reserve bottle for a less-than-full pressure. Pressures of full bottles in the table below.

345 & 366:

Safety cupboards are fitted with an audible and visual alarm when one bottle is empty but not all alarms are working! Switching over to the full bottle occurs automatically.

366:

All gas supplies in room 154

Switching over to the full bottle(s) occurs automatically. However taps must be closed and remaining pressure released to allow removal of the empty bottles

Alarm when all bottles are empty (11.2023: alarm currently not working!), or when there is a gas leak.

In case of gas leak, open door but do not enter until alarm stops.

In case of fire alarm, the solenoids in the gas line switch off. Reopen with the 'Reset' command on the controller box.

Instructions:

To remove the empty bottle:

rotate the handle so the 'Entnahme' arrow points to the currently in-use bottle;
turn the tap on the top of the empty bottle to close it;
release the remaining pressure in the pipe with the knob marked 'Restgase';
close the 'Restgase' knob;
undo the nut holding the pipe using the spanner(s) (some non-CO₂ bottle nuts have a reverse thread!); do not lose the sealing ring that goes between the bottle and the pipe - it belongs on the end of the pipe. Spares: see table below;
screw a bell-shaped protective cover over the tap;
The bottle can be removed and loaded into the gas bottle trolley.

To connect a new bottle:

remove the 'Vollgut' tape around the protective cover and unscrew the bell-shaped protective cover;
pull off the yellow plastic cover over the connection thread;
turn the bottle so that the connection faces the end of the pipe;
make sure there is a seal on the end of the pipe, connect the tube and do up the nut firmly but not over-tight;
undo the tap on the top of the bottle a few turns until the pressure gauge climbs or you hear the gas entering the system. Do not open the tap the maximum amount;
listen for the hissing of escaping gas and if necessary do up the nut a little tighter until the hissing stops;
Finished.

Full bottles of Bading CO2 and synthetic air mixtures are obtained from the Gasflaschenlager.

Empty Bading CO2 and synthetic air mixtures bottles go to the Gasflaschenlager.
CO2 4.5% + N 5% bottles are stored

Full bottles of Schuster-CO2 and Carbogen and are obtained from the Gasflaschenlager only.
Empty Schuster CO2 bottles go to the Gasflaschenlager only.

Full bottles are marked with a 'Vollgut' tape around the screw-on protective cover.

The gas bottle trollies (1 single-bottle, 1 double-bottle) are in the gas bottle room 154.

It is forbidden for anyone to travel in the lift with a gas bottle (even with an 'empty' bottle); put the bottle in the lift, pull the red/white tape (if available) across the lift doorway, send the lift to its destination and go there via the stairs or other lift.

All other bottles go to the Gasflaschenlager.
Put the receipt for the returned empty bottle under 'G' in the receipt folder opposite 104.
Receipts for empty Schuster bottles go to Christoph Schuster.

Always bring back or order* a replacement bottle. A Theoretikumskarte (Neurobiologie or Schuster – see table below) is required.
* CO₂ is always available; the CO₂/Synthetic air mixture has to be ordered, delivery 3-6 weeks - you will be informed of delivery by telephone.

Access to the Gasflaschenlager is either overground or underground to 346 and then overground.
Gasflaschenlager location and opening times here.

Location

Type

Contents

Full pressure @ room temperature

Notes

Replacement

345

Corridor, opposite lab 220

Cupboard

CO₂

50 bar

11.2023: Bottle alarm inactive

Responsible: Christoph Schuster

Gasflaschenlager + Schuster group's Theoretikum card. Ask Emilia (221) or Christoph (205) for the card.

Carbogen:
95% O₂ + 5% CO₂

150 bar

11.2023: Bottle alarm inactive

Responsible: Peter Bengtson

Gasflaschenlager + Neurobiologie Theoretikum card. Ask in 157 (Spülküche), 102 or 103 for the card.

Lab 222a

Free-standing

–

Responsible: –

Corridor, opposite room 243

Cupboard

CO₂

50 bar

Bottle alarm OK?

Tools kept here.

Corridor 293b

Free-standing

Compressed air for confocal microscope table

Responsible: Peter Bengtson

Location

Type

Contents

Notes

Replacement

366

Room 154

Room

O₂

–

CO₂

200 bar

–

50 bar

11.2023: Bottle alarm inactive

Tools and spare sealing rings kept here.

Some bottles in pairs.

Gasflaschenlager + Neurobiologie Theoretikum card. Ask in 157 (Spülküche), 102 or 103 for the card.

Liquid nitrogen for refillable tank in INF366 room 150

The display (press the red button) will show 70-80 when full (display currently broken!). Ideally it should show more than 20 to be sufficiently full for a weekend but can be empty for a few days without damaging the frozen cells.

Refilled once a week (Thursday/Friday).

To disconnect the tank:

wear ear protection or put fingers in ears and close the door – the next step is LOUD!
release any pressure in the tank by opening the unconnected lever near the neck. Don't stand in the path of the pressure release outlet!
wearing gloves, remove the locking collar from the neck
wearing gloves, withdraw the long tubes and lean them against the wall
wearing gloves, remove the metal ring and silicon ring from the neck opening
drop the stopper into the neck of the tank
unlock the wheel(s).
the tank is now free to move.

Gas store location and opening times here.

Take the tank to be refilled to the gas store early so the full tank can be collected during the opening time. After the opening time, the tank will have to be collected by asking at the Zentrallager checkout.

Theoretikumskarte is not normally required, but a receipt has to be signed. The copy goes into the Lieferscheine book under 'G' ('Gases').

The full tank might be too heavy for the small lift. In the past it occasionally caused the lift to stop.

To connect the tank:

lock a wheel
wearing gloves, place the sealing ring onto the neck opening
wearing gloves, re-insert the long tubes – very slowly to avoid boiling the liquid nitrogen. Don't stand in the path of the pressure release outlet!
re-attach and tighten the locking collar to the neck
close the pressure-release tap.

Glassware

Lab glassware is stored in the glass cupboards in 366 and in the steel cupboard in front of lab 221 (345).

Cell culture glassware is separated from normal lab glassware and should not be mixed.

It is stored in the glass-cupboards in front of the cell culture facility in 366 and in front of the lab 221 in 345.

You should never!! add anything to the bottles containing sterile water and 1x PBS.

Sterile water and 1x PBS should be used for tissue culture work only, without addition of anything.
The water from the Millipore device is sterile filtered but not autoclaved, which means for all lab purposes sufficient.

If you contaminate the water and 1xPBS bottles with any chemicals you risk our tissue culture cells.

Holidays

Before you go on holiday make sure that you have a substitute for your responsibilities.

You have to fill a holiday form, get the signature of your group leader and give it to the Secretary, for reasons of insurance.

Housing rooms

The lighting is controlled by emote access to the Zentrale Leittechnik der Uni-Heidelberg:

https://129.206.91.242/cgi-bin/welcome

Accept the risks of entering a website without a trustworthy certificate

Password: known to Ana Oliveira and Anna Hertle

Domain: unihd

Click the button

A new window opens

Click the 3 horizontal lines to get the sub-menu and under Datenpunktliste, double-click the room number and under Parameter you can see and change the on/off times.

Hygiene Plan S1 / S2

Hygiene Plan S1 Deutsch And English

Hygiene Plan S2 Deutsch And English

IncuCyte Smart Cell Monitoring & Analysis

The IncuCyte machine is operating in room 143

Software module guidelines:
Angiogenesis-Analysis.pdf
ATP-Analysis.pdf
Basic-Analysis.pdf
Cell-by-Cell-Analysis.pdf
Chemotaxis-Analysis.pdf
Neuronal-Activity-Analysis.pdf
Neurotrack-Analysis.pdf
Organoid-Analysis.pdf
Scratch-Wound-Analysis.pdf
Spheroid-Analysis.pdf

Windows

IncuCyte machine IP address: 129.206.50.20

Guest name and password: essenbio

Standard user accounts from Katja Baur or IT_adminitstrator

Live-cell imaging and analysis

Device serial number IC51426

Device MAC address ac1f6bdbf38a

Battery backup power for ca. 20 minutes

Client download from the Nbio software page

Remote access (also from home using VPN) available

See this intranet page about Remote Access and VPN

Isotope Lab

Before you are allowed to work with isotopes contact the Radioactivity Safety Officer - Anna Hertle

K12 Bacteria

K12 Bacteria Rules and Regulations: K12 Bacteria German K12 Bacteria English

Keys and magnetic cards

Keys for lockers and magnetic cards for the main doors and isotope labs are available from the Secretary.
You have to leave a deposit of €25.

Last person's duties

If you are the last person who leaves the lab make sure that

- there is no gas burner working

- the hoods are switched off

- the microscopes are switched off

- centrifuges that are not in use overnight are switched off and the lids open

- water-baths and magnetic stirrers (especially heatable ones) and heat blocks that are not in use overnight are switched off

- the Ultrospecs are switched off

- all windows are closed

- the Institute doors are properly locked.

Leica CM1950 Cryostat

instructions and tutorials here.

Library

Universitätsbibliotek Zweigstelle INF368

Copy card available from the Secretary.

MEA Multi-Electrode Array

http://www2.nbio.uni-heidelberg.de/intranet/MEA/MEAsetup.html

Media for Bacteria culture

LB-Medium and L-Agar is stored in the glass cupboard next to lab ?.

Mineral water

is available for €0.70 per bottle in the printer/copier room 146. Don't forget to pay and bring back your empties.

Nitrogen tanks for storage of cells

The space available is very limited, so store only those samples in liquid nitrogen that have to be. The columns in the storage tank are distributed to the groups. Use cryotubes only and label them. List in the cryostorage folder of your group what you store in liquid nitrogen. If you need additional space ask Ursula Weiß.

Nitrogen tanks for storage of cells

Office supplies

are stored in the cupboard opposite the Secretary's office. If you need something contact the Secretary's office.

PCR

ThermoFischer Applied Biosystems 96-well SmartOneStep plus Real-Time PCR System
Room 142

Product page
Software downloads
Instruction manuals in a drawer in ?
Original software in room 204

pH Meter

Room ?, FiveEasy F20 Handbook

Phosphor Imager

Not currently in use. Stored in 346/U01a.

PI_info.html

Plate Reader

CLARIOstar Plus

Responsible: Priit Pruunsild

Repairs

Water, electricity, air-conditioning, heating, elevators, blocked toilets

If you find any problems call the Störungsannahmestelle 5114, give the building and room number, your name and phone number and describe the problem, in German preferably.

Repair of scientific equipment should be initiated by the person in charge: Responsibilities in collaboration with Anna Hertle.

Responsibilities

https://www.nbio.uni-heidelberg.de/intranet/equipmentresponsibilities.html

Safety: S1 & S2

Everyone who works in the lab (also students) must be introduced to S1 rules by the person in charge (Anna for the Bading group, Francesca for the Ciccolini group, Christoph for the Schuster group, Ana for the Oliveira group and Daniela for the Mauceri group) and must sign the respective S1 book.

If S2 work is planned, a medical examination by the Betriebsarzt is obligatory. Anna Hertle gives the S2 introduction and the S2 book has to be signed.

Labeling bench aliquots: See "Lab Safety" Section of Anna's SOPs for a key to the correct labels to use (Password = "Science!"): https://1drv.ms/u/s!Ai6uLdHu6cZ9dMLpLG8xUnPnmhE?e=uI7g2U.

Safety movies here

Journals and books

should be taken out only for copying. Put them back on the shelves after use. If you need a book log in to the book folder on the book shelf with your name and the title and number of the book.

Coffee kitchen

Put everything (cups, coffee, spoons etc.) you have used back in the cupboards. Dirty cups and dishes should be put in the dish washer. Keep everything clean. Empty the dish washer when it has finished.

Fridge

All items which are stored in the fridge have to be labelled with the owners name. Unlabelled things will be thrown away

SnapGene

SnapGene software is installed on a PC in room 152 (PCR).

Download

macOS: Requires macOS 10.14 (Mojave) or higher
Windows: Requires Windows 10 or higher

See this intranet page about Remote Access and VPN

Spectrophotomer, see

Telephone and fax use

The fax machine is in the Sekretariat.

For connections outside the Uni (non 06221 54- numbers) dial a zero first, except

connections to DKFZ (42-xxxx) dial 116-xxxx

connections to Klinik (56-xxxx) dial 114-xxxx

For private calls or faxes dial 90 (instead of zero) and list your call or fax in a list next to the phone/fax you are using. A bill with costs and numbers of private calls will be distributed to the labs.

Telefon-Handbücher

Telephone lists

Private

https://www.nbio.uni-heidelberg.de/intranet/contact.html

The telephone list with department and private telephone numbers is not accessible outside the Department.

Public

The public Neurobiologie home page has the same list without the private telephone numbers.

Please inform the Secretary of any change of your address or telephone number.

Vacuum pumps

Rotary pumps / Drehschieberpumpen

These pumps are maintained by the Lab. Kitchen staff.

Membrane pumps / Membranpumpen

The pumps in the tissue culture rooms have bottles which have to be emptied before you start.

Waste disposal

Chemical waste

is usually collected by the Lab. Kitchen staff and brought to the waste disposal department www.zbt.uni-heidelberg.de/chemie/

If a waste canister is full you must

- close it properly

- clean the outside properly

- put a correctly labelled waste description form into the transparent plastic pocket

- fill in the yellow sheet (Abfallbegleitschein) according to example

Special chemical waste

needs special treatment and is collected in special containers:

Ethidiumbromide liquid: 5 litre plastic cannister

Ethidiumbromide solid (agarose gels, gloves): blue container, Lab 120

Acrylamide gels, contaminated consumables: blue container, Lab 120

X-ray film fixer: 12 litre plastic cannister

X-ray film developer: 12 litre plastic cannister

Organic solvents without halogens (Comassi stain sol.): 5 litre plastic cannister

Organic solvents with halogens (Phenol/Chloroform etc.): 5 litre plastic cannister

used vacuum-oil (has to be checked for radioactivity): 5 litre plastic cannister

If you exchange any cannister try to use one which had the same content before and which is labeled already.

Glass waste

is collected in two different ways:

Lab glass

Pasteur pipettes and Schott glass, broken lab bottles, Erlenmeyer etc. is collected and disposed of in the glass container outside building 367 by the Kitchen staff.

Normal glass

Glass, bottles etc. can be taken to the glass container outside building 367 for recycling.

Bacterial waste

Solid

is collected in autoclave bags (available in tissue culture pre-room) and has to be put in the cleaning kitchen, left side of the big autoclave.

Lab waste

used disposable lab items like tissue, plastic tubes etc., which have not been in contact with GVOs (= 'genetically changed organisms'), should be put into the normal lab waste or Restmüll bins.

Paper

goes into the green paper bins.

Cardboard boxes

should be flattened and put into the transport wagon near the lift in 366 or opposite lab 207 in 345.

Polystyrene boxes

Some companies such as Fermentas, Promega, Roche take them back via their transport companies. Treat them according to the company's rules. These boxes are stored in the area near the lift in 366 or in the west corridor in 345.

Clean plastic and metal

goes into the yellow bins.

CDs and DVDs

The discs and cases can go into the 'Restmüll' bins, the paper inserts into the green 'Papier' bins.

Batteries

go into the green collection box opposite the Uni. Shop, INF 306 or into the box in 204. Rechargeable batteries are also collected in 204.

Overview

An overview of the waste collection on the campus is here.

Workshops

Electronics workshop

http://www.zbt.uni-heidelberg.de/elektronik/index.html

Glass workshop

http://www.zbt.uni-heidelberg.de/glastechnik/index.html

Mechanical workshop

http://www.zbt.uni-heidelberg.de/feinmechanik/index.html

To have something made or repaired, you need the Theoretikum card of your group. For repairs clean the item and if it could have been exposed to radioactivity, check whether it is radioactively contaminated (with the signature of the Radioactivity Safety Officer if necessary).

ZEISS Microscope Camera

Updated: 22.02.2022

The Axiocam ERc 5s camera in room 142 is controlled by a laptop using ZEISS Labscope for Windows.

Save images either to a USB stick or to the Backup server's ZEISS folder ** (alias on the desktop). The files on the Server and are then accessible on the Neurobiology intranet by accessing the Shared Folder (User: ZEISS Password: Axiocam ) by the following methods:

Mac: click this link smb://Backup/
Windows: File Explorer / Network open Backup/
Ubuntu: Files / Other Locations, enter smb://Backup/ in the search field.

** Folders cannot be created by users, so contact the IT administrator and ask for one.

Files on the laptop itself are not backed up!

Labscope Users Guide download

Portal.ZEISS.com for downloads and support.
User: ITadmin@nbio.uni-heidelberg.de Password: Zeiss69120#

ZEISS Axiophot microscope

The ZEISS Axiophot microscope in room 155 has a BASLER ace camera and BASLER microscopy image acquisition software.

µManager open source software is compatible but not installed.

If you have any suggestions to improve this lab guide send an e-mail to ITadmin@nbio.uni-heidelberg.de

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Contact

Lab Handbook

If you are the last person to use this facility

How to use the cooling- and ultra-centrifuges and rotors

Maintenance of centrifuges

Photocopiers/scanners in 345 & 366

University library

DKFZ

Room 150

Room 154

Re-opening the gas supply valves (also after a fire alarm, when the valves will have been turned off automatically)

On the touch screen next to the department's main door, push the flashing warning symbol at the top and ACK ('Acknowledge', confirm) to reset the alarm at the Leitwarte.

Bottled gases

Liquid nitrogen for refillable tank in INF366 room 150

Journals and books

Coffee kitchen

Fridge

Private

Public

Rotary pumps / Drehschieberpumpen

Membrane pumps / Membranpumpen

Chemical waste

Glass waste

Lab glass

Normal glass

Bacterial waste

Lab waste

Paper

Cardboard boxes

Polystyrene boxes

Clean plastic and metal

CDs and DVDs

Batteries

Overview

Electronics workshop

Glass workshop

Mechanical workshop

Re-opening the gas supply valves
(also after a fire alarm, when the valves will have been turned off automatically)